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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
09/11/2017 |
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Actualizado : |
12/12/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
FEDERICI, M.; BAJSA, N.; LAGURARA, P.; REVALE, S.; MARCONDES, J.A.; DALLA RIZZA, M. |
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Afiliación : |
MARIA TERESA FEDERICI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; NATALIA BAJSA VALVERDE, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); PAULA LAGURARA, MEC/ IIBCE (Instituto de Investigaciones de Ciencias Biológicas "Clemente Estable"); SANTIAGO REVALE, INDEAR (Instituto de Agrobiotecnología de Rosario); JACKSON A. MARCONDES, Universidad Estadual Paulista (UNESP)/ Campus de Jaboticabal; MARCO DALLA RIZZA VILARO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Soil and rhizosphere bacterial diversity in maize agro-ecosystem. |
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Fecha de publicación : |
2017 |
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Fuente / Imprenta : |
Sustainable Agriculture Research, 2017, v.6 (3): 35-51. |
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ISSN : |
1927-050X (Print) // 1927-0518 (Online) |
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DOI : |
10.5539/sar.v6n3p |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017. |
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Contenido : |
ABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices.
Copyright © Canadian Center of Science and Education MenosABSTRACT.
Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial ... Presentar Todo |
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Palabras claves : |
DGGE; METAGENOMICS; PYROSEQUENCING; RHIZOSPHERE; SOIL BACTERIA; TRANSGENIC MAIZE. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/7925/1/67345-252782-1-PB.pdf
http://www.ccsenet.org/journal/index.php/sar/article/view/67345/37668
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Marc : |
LEADER 02541naa a2200289 a 4500
001 1057747
005 2018-12-12
008 2017 bl uuuu u00u1 u #d
022 $a1927-050X (Print) // 1927-0518 (Online)
024 7 $a10.5539/sar.v6n3p$2DOI
100 1 $aFEDERICI, M.
245 $aSoil and rhizosphere bacterial diversity in maize agro-ecosystem.$h[electronic resource]
260 $c2017
500 $aArticle history: Received: April 3, 2017; Accepted: May 20, 2017; Online Published: June 16, 2017.
520 $aABSTRACT. Management practices used in maize production have an impact on soil agro- ecosystems where different microbial communities coexist. Soil inhabiting bacteria are numerous and diverse, but we know very little about their ecological distribution. Here we analyzed the bacterial community diversity in the rhizosphere of two transgenic maize cultivars, in agricultural soil before sowing and in non-cultivated soil in an experimental site in the south region of Uruguay. We followed two culture-independent methods: DGGE (denaturing gradient gel electrophoresis) and 454-pyrosequencing of 16S rRNA gene amplicon. Through pyrosequencing, the three environments analyzed presented differences in terms of bacterial composition. However, no differences were found in the relative abundance of the ten most represented phyla in the rhizosphere of the two cultivars at different phenological stages. We found significant differences of Bacteroidetes, Gemmatimonadetes, Planctomycetes, Proteobacteria and Verrucomicrobia phyla when comparing agricultural and non-cultivated soils, as well as a significant enrichment of members of the phylum Gemmatimonadetes in all rhizosphere samples compared to soil. Through DGGE analysis we evidenced that maize rhizosphere bacterial communities changed at different phenological stages in both cultivars. We also provided baseline information about bacterial specific taxa within maize agro- ecosystem for further evaluation of possible rhizosphere bacterial community shifts of genetically modified maize cultivars under different management practices. Copyright © Canadian Center of Science and Education
653 $aDGGE
653 $aMETAGENOMICS
653 $aPYROSEQUENCING
653 $aRHIZOSPHERE
653 $aSOIL BACTERIA
653 $aTRANSGENIC MAIZE
700 1 $aBAJSA, N.
700 1 $aLAGURARA, P.
700 1 $aREVALE, S.
700 1 $aMARCONDES, J.A.
700 1 $aDALLA RIZZA, M.
773 $tSustainable Agriculture Research, 2017$gv.6 (3): 35-51.
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Registro original : |
INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha actual : |
31/08/2016 |
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Actualizado : |
05/11/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CALVO-SALAZAR, V.; SINGH, R. P.; HUERTA-ESPINO, J.; CRUZ-IZ QUIERDO, S.; LOBATO-ORTIZ, R.; SANDOVAL-ISLAS, S.; VARGAS-HERNÁNDEZ, M.; GERMAN, S.; SILVA, P.; BASNET, B. R.; LAN, C. X.; HERRERA-FOESSEL, S. A. |
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Afiliación : |
SILVIA ELISA GERMAN FAEDO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA PAULA SILVA VILLELLA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Genetic Analysis of Resistance to Leaf Rust and Yellow Rust in Spring Wheat Cultivar Kenya Kongoni. |
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Fecha de publicación : |
2015 |
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Fuente / Imprenta : |
Plant Disease, v. 99, no.1153-1160, 2015. |
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ISSN : |
0191-2917 |
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DOI : |
10.1094/PDIS- 07-14 -0718-RE |
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Idioma : |
Inglés |
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Notas : |
Article history: Accepted for publication 9 February 2015/Published Online:23 Jun 2015. |
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Contenido : |
Abstract: The Kenyan wheat (Triticum aestivum L.) ?Kenya Kongoni ?exhibits high levels of adult plant resistance (APR) to leaf rust (LR) and yellow
rust (YR). We determined the genomic regions associated with LR and YR resistance in a population of 148 recombinant inbred lines generated
from a cross between ? Avocet-YrA ?and Kenya Kongoni. Field experi-ments to characterize APR to LR and YR were conducted in four and
two Mexican or Uruguayan environments, respectively. A linkage map was constructed with 438 diversity arrays technology and 16 simple-
sequence repeat markers by JoinMap 4.1 software. Genetic analyses showed that resistance to both rusts was determined by four to five
APR genes, including Lr46/Yr29 and Sr2/Lr27/Yr30 . Quantitative trait loci (QTL) analysis indicated that pleiotropic APR loci QYLr.cim-1BL
corresponding to Lr46/Yr29 and QYLr.cim-7BL that is a putative novel QTL accounted for 5 to 57% and 12 to 35% of the phenotypic variation
for resistance to LR and YR, respectively. These loci, in combination with another three LR QTL and two YR QTL, respectively, conferred
high levels of resistance to both LR and YR in wheat under Mexican and Uruguayan environments. Among ot her detected QTL, QLr.cim-1DS,
QLr.cim-2BL, and QYLr.icm-7BL may be new loci for APR to both rusts in common wheat. |
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Thesagro : |
ENFERMEDADES DE LAS PLANTAS; PUCCINIA GRAMINIS; PUCCINIA STRIIFORMIS. |
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Asunto categoría : |
H20 Enfermedades de las plantas |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13737/1/Plant-Disease-v.-99-no.1153-1160-2015..pdf
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Marc : |
LEADER 02362naa a2200325 a 4500
001 1055482
005 2019-11-05
008 2015 bl uuuu u00u1 u #d
022 $a0191-2917
024 7 $a10.1094/PDIS- 07-14 -0718-RE$2DOI
100 1 $aCALVO-SALAZAR, V.
245 $aGenetic Analysis of Resistance to Leaf Rust and Yellow Rust in Spring Wheat Cultivar Kenya Kongoni.$h[electronic resource]
260 $c2015
500 $aArticle history: Accepted for publication 9 February 2015/Published Online:23 Jun 2015.
520 $aAbstract: The Kenyan wheat (Triticum aestivum L.) ?Kenya Kongoni ?exhibits high levels of adult plant resistance (APR) to leaf rust (LR) and yellow rust (YR). We determined the genomic regions associated with LR and YR resistance in a population of 148 recombinant inbred lines generated from a cross between ? Avocet-YrA ?and Kenya Kongoni. Field experi-ments to characterize APR to LR and YR were conducted in four and two Mexican or Uruguayan environments, respectively. A linkage map was constructed with 438 diversity arrays technology and 16 simple- sequence repeat markers by JoinMap 4.1 software. Genetic analyses showed that resistance to both rusts was determined by four to five APR genes, including Lr46/Yr29 and Sr2/Lr27/Yr30 . Quantitative trait loci (QTL) analysis indicated that pleiotropic APR loci QYLr.cim-1BL corresponding to Lr46/Yr29 and QYLr.cim-7BL that is a putative novel QTL accounted for 5 to 57% and 12 to 35% of the phenotypic variation for resistance to LR and YR, respectively. These loci, in combination with another three LR QTL and two YR QTL, respectively, conferred high levels of resistance to both LR and YR in wheat under Mexican and Uruguayan environments. Among ot her detected QTL, QLr.cim-1DS, QLr.cim-2BL, and QYLr.icm-7BL may be new loci for APR to both rusts in common wheat.
650 $aENFERMEDADES DE LAS PLANTAS
650 $aPUCCINIA GRAMINIS
650 $aPUCCINIA STRIIFORMIS
700 1 $aSINGH, R. P.
700 1 $aHUERTA-ESPINO, J.
700 1 $aCRUZ-IZ QUIERDO, S.
700 1 $aLOBATO-ORTIZ, R.
700 1 $aSANDOVAL-ISLAS, S.
700 1 $aVARGAS-HERNÁNDEZ, M.
700 1 $aGERMAN, S.
700 1 $aSILVA, P.
700 1 $aBASNET, B. R.
700 1 $aLAN, C. X.
700 1 $aHERRERA-FOESSEL, S. A.
773 $tPlant Disease$gv. 99, no.1153-1160, 2015.
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